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. 2008 Jul 25;95(9):4372–4383. doi: 10.1529/biophysj.108.135103

FIGURE 1.

FIGURE 1

Structure of an HJ. (a) Diagram outlining the principle of immobile-HJ folding used in single-pair FRET studies. (b) Schematic of the HJ central sequence with a 20-bp D-A distance, including Cy3 (donor) and Cy5 (acceptor) dyes marked by arrows. Variable parts of the HJ design are shown in boldface letters (see Materials and Methods for details).