FIGURE 4.

Survival and maturity of glial cell types are modestly affected by substrate modulus. (A) Number of attached cells on various substrates, differentiated under glial media conditions (1 v/v % fetal bovine serum), black in all plots. In all plots, dark gray is laminin-coated polystyrene. Shown also are control conditions with neuronal media on laminin-coated polystyrene substrates (1 μM retinoic acid + 5 μM forskolin; no serum), which are light gray in all plots. (B) Histograms indicate number of cells at particular GFAP staining intensities. All images were taken after 6 days of culture. (C) Summary of A. Number of attached cells as a function of modulus at 4 h after seeding, and after 6 days of differentiation. (D) Summary of B. Percentage of cells that stained positive for GFAP for each intensity bin. For A, C, and D, error bars are 95% confidence intervals. Means with same group letter are not significantly different from each other (analysis of variation Tukey-Kramer test, p < 0.05). Note the log x-axis in C and D.