Table 1.
The primers used for cloning P450 genes, qRT-PCR reactions, and SNP determinations
| Gene | Primer Name | Function | Primer Sequence | Position (nt) |
| CYP4G2 | AP450HF20F | Full length cloning | 5' ATGGATAGTGCAAACAATTCAACTGCG 3' | 1 to 27 |
| SNP450HF20F | SNaP determination | 5' AAATGGCCATTTGGTGGCCCAT 3' | 174 to 195 | |
| RTP450HF20F | qRT-PCR | 5' CGAGGAGGATGATGAAATAAGCAAGC 3' | 837 to 862 | |
| RTP450HF20R | qRT-PCR | 5' TTGGACATGGCCATCATGGCATCT 3' | 957 to 980 | |
| P450HF20R | 5'-RACE | 5' GCAGGAAGTTGTCACCAAAGATG 3' | 1140 to 1162 | |
| P450HF20F | 3'-RACE | 5' CGTGCATCGCAATCCCCAATAC 3' | 1341 to 1362 | |
| AP450HF20R | Full length cloning | 5' TTACATAGCTTTCATGGCTTCGGGTC 3' | 1625 to 1650 | |
| CYP4D4v2 | AP450HF5F | Full length cloning | 5' ATGTTATTTGAATTCCTGGTGGGTC 3' | 1 to 25 |
| SNP450HF5F | SNaP determination | 5' ACATGACACCACGACAAGTGG 3' | 948 to 968 | |
| RTP450HF5F | qRT-PCR | 5' AGGATAAGGAGAAACCGGTGACC 3' | 1052 to 1074 | |
| RTP450HF5R | qRT-PCR | 5' CAATTGTCGGCACCGATGGATAC 3' | 1156 to 1134 | |
| P450HF5R | 5'-RACE | 5' AGCAACTCAAAATGGCGTACC 3' | 1430 to 1410 | |
| AP450HF5R | Full length cloning | 5' TAACTACTTGCGAACTCTCAAACCC 3' | 1521 to 1497 | |
| CYP6A38 | P450HF17P-F | 5' flanking region cloning | 5' TGGTCTTCTAGGGGAGAAGACTACCTGC 3' | -676 to -649 |
| SNP450HF17PF | SNaP determination | 5' TTCAGGATTGCTGGGTAGCT 3' | -69 to -50 | |
| P450HF16F-3 | Full length cloning | 5' CATTATGGAGACTTCGGGAGTTTTG 3' | -4 to 21 | |
| P450HF17P | 5' flanking region cloning | 5' CGTAGGTTCCTCATGGGGTATACCCAGC 3' | 120 to 93 | |
| RTP450HF17F | qRT-PCR | 5' CCCTGATGGGCAACATGAATGGAT 3' | 122 to 145 | |
| RTP450HF17R | qRT-PCR | 5' TAGTTGTTTGTCCAGCAGCACCAC 3' | 270 to 247 | |
| P450HF17R | 5' RACE | 5' CGCTGTACTTCAATAGATTTCCTGC 3' | 650 to 626 | |
| P450HF17F | 3' RACE | 5' TGAGGGTGATACAAAACCAAGC 3' | 1017 to 1038 | |
| AP450HF17R | Full length cloning | 5' GAGATAATCTCCCACCCCTAAATCG 3' | 1520 to 1496 | |
| Common | Flyh1 | 5' GGICCIAGIAACTGCATIGG 3' | ||
| Flyc1 | 5' GGAAGTNGACACNTTYATGTT 3' | |||
| CYP6AD1 | 5' GTNATHGGHHNBTGYGCHTTYGG 3' | |||
| HemeR1 | 5' CCIATGCAGTTICTIGGICC 3' | |||
| Oligo (dt) | 5' TAATACGACTCACTATAGGGAGATTTTTTTTTTTTTTTT 3' | |||
| C2 | 5' TAATACGACTCACTATAGGGAGA 3' | |||
| AP1 (RACE) | 5' CCATCCTAATACGACTCACTATAGGGC 3' | |||
| AP1 (GenomeWalking) | 5' GTAATACGACTCACTATAGGGC 3' | |||
| ActinS1 | 5' AGGCGAATCGCGAGAAGATG 3' | |||
| ActinAS1 | 5' TCAGATCACGACCAGCCAGATC 3' | |||