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Chondrogenic differentiation of ATDC5 cells by Smads. (A) ATDC5 cells were infected with adenoviruses carrying Smad1–Smad7 at an m.o.i. of 300 (iii–ix). Cells were stained by Alcian Blue 8 d after infection. Adenovirus with β-galactosidase (m.o.i. of 300) was used as a control (ii). (B) Incorporation of [35S]sulfate into ATDC5 cells by Smad1–Smad7 was determined 10 d after infection.
