Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Oct 20;183(2):187–194. doi: 10.1083/jcb.200805185

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Yamamoto et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Molecular dependency of kinetochore localization of SPDL-1 and other kinetochore proteins. (A) Embryos dissected from adult hermaphrodites untreated (WT) or injected with dsRNA of indicated genes were fixed and analyzed by immunofluorescence microscopy. Images of embryos at metaphase of the first mitosis are shown. KNL-1 and CZW-1 are required but NDC-80 or BUB-1 are not for SPDL-1 targeting to kinetochores. Bar, 20 μm. (B) Lysate from wild-type worms untreated (WT) or injected with dsRNA of indicated genes were analyzed by Western blotting with antibody to indicated proteins. To each lane, whole-worm lysates from 20 injected worms was loaded. (C) Embryos expressing indicated protein fused with GFP were dissected from adult hermaphrodites untreated (WT) or injected with spdl-1 dsRNA and analyzed by time-lapse fluorescence microscopy. Images of embryos at metaphase of the first mitosis are shown. For BUB-1, wild-type embryos were dissected from wild-type adult hermaphrodites untreated (WT) or injected with spdl-1 dsRNA, fixed, and stained with anti–BUB-1 antibody. Kinetochore localization of proteins was not affected in SPDL-1–depleted embryos. Bars, 10 μm.