Table I. Analysis of NB mitosis and cell cycle.
| Genotype | Duration of mitosis NEB–anaphase onset |
Cell cycle length NEB–NEB |
|---|---|---|
| min/s | min | |
| Wild-type clones | 7:41 ± 3:05 (n = 26) |
73 ± 16 (n = 11) |
| APC2d40 APC1q8 | 9:39 ± 4:13a (n = 21) |
87 ± 20 (n = 9) |
| Wild-type clones | 8:51 ± 3:29 (n = 24) |
79 ± 41 (n = 11) |
| APC2g10 APC1q8 | 11:33 ± 4:03bc (n = 35) |
127 ± 24bd (n = 14) |
All measurements include only the first and second imaged cell cycles per cell to reduce possible ambiguous results caused by light damage or prolonged culturing. NEB, nuclear envelope breakdown. n, number of clones analyzed.
a
P < 0.05 when compared with wild-type controls.
b
P < 0.01 when compared with wild-type controls.
c
P < 0.05 when compared with APC2d40 APC1Q8.
d
P < 0.001 when compared with APC2d40 APC1Q8.