Table 3. Effect of VHHSI14 on natural TcTS from T. cruzi trypomastigotes.
| TcTS activitya | |||
| Total activity | Preincubated with VHHSI14 (40 ng) | Preincubated with lactitol 1 mMb | |
| Recombinant TcTS611/2c | 3279±314 | 621±212 (81.1) d | 422±218 (87.1) |
| Natural TcTSe | |||
| Cl-Brener strain | 1154±135 | 1017±71 (11.9) | 188±33 (83.7) |
| RA strain | 1653±184 | 1576±162 (4.6) | ND f |
| Villegas strain | 1960±90 | 1562±145 (22.8) | 514±70 (73.7) |
| Natural TcTS plus recombinant TcTS | |||
| Supernatant (Cl-Brener) | 2318±288 | 2082±272 (10.2) | ND |
| Recombinant TcTS611/2 | 3286±401 | 651±201 (80.1) | ND |
| Supernatant (Cl-Brener) plus recombinant TcTS611/2 | 5134±109 | 2461±466 (52.1) | ND |
| Cl-Brener trypomastigotes g | 918±168 | 834±102 (4.8) | ND |
| Inmunoprecipitated TcTS (Cl-Brener) g | |||
| Treated with PNGasa | 2632±396 | 2411±450 (9.2) | 298±198 (88.7) |
| Untreated with PNGasa | 2157±237 | 2079±243 (3.6) | 321±184 (85.1) |
a
Results are expressed in CPM (Counts Per Minute) obtained after 1 hour of reaction (by TIA). The values are the mean and standard deviation of at least three independent experiments.
b
Lactitol was assayed as positive inhibition control on Natural TcTS.
c
Recombinant TcTS was assayed as positive control of inhibition in every single test.
d
Parenthesis indicate percentage of inhibition of TcTS activity that remnant CPM represents .
e
Supernatant from cell culture derived trypomastigotes were used.
f
ND: not determinated.
g
Decameric form of VHHS14 was used.