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The sxi1α SXI2a and the sxi2a SXI1α strains are sterile when crossed to a or α cells but are interfertile. (A) Strains of the indicated genotype were crossed to JEC20 and JEC21 on MS medium and photographed after 5 days of incubation. Aberrant filaments but no basidiospores were observed. (B) Two strains of the indicated genotypes were cocultured on MS medium and incubated for 5 days in the dark at 25°C. Abundant hyphae and basidiospore chains were seen at the edge of the mating colony.
