FIG. 6.

Exchanging the homeodomain proteins does not alter uniparental mitochondrial inheritance. Progeny were isolated from the cross sxi1α SXI2a (YPH716, mitochondria type I) × sxi2a SXI1α (YPH227, mitochondria type II) or α (H99, mitochondria type I) × “a” (JF289 mitochondria type II) and typed for mitochondrial inheritance. All progeny inherited the type II mitochondria from the a parent. Nuclear markers were also scored by drug resistance, PCR and mating assays. “+” indicates the sxi1α or the sxi2a mutants, which are NEO or NAT resistant, and also the presence of the SXI1α -URA5 or SXI2a-URA5 transgenes, identified by PCR analysis. Mating assays were conducted to score the wild-type SXI1α allele in the MAT locus.