TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| C. neoformans var. grubiia | ||
| H99 | MATα | 28 |
| KN99a | MATa | 24 |
| F99 | MATα ura5 | 24 |
| JF99a | MATaura5 | This study |
| JF135 | MATα sxi1α::NEO ura5 | This study |
| JF271 | MATasxi2a::NAT ura5 | This study |
| JF306 | MATα sxi1α::NEO ura5 SXI1α::URA5 | This study |
| JF289 | MATasxi2a::NAT ura5 SXI2a::URA5 | This study |
| YPH153 | MATaSXI1α::URA5 (MATaSXI1α) | This study |
| YPH510 | MATα SXI2a::URA5 (MATα SXI2a) | This study |
| YPH227 | MATasxi2a::NAT ura5 SXI1α::URA5 | This study |
| YPH220 | MATα sxi1α::NEO ura5 SXI2a::URA5 | This study |
| YPH716 | MATα sxi1α::NEO ura5 SXI2a::URA5 (H99 mitochondria) | This study |
| C. neoformans var. neoformans | ||
| JEC20 | MATa | 19 |
| JEC21 | MATα | 19 |
| Plasmid | ||
| pJAF7 | URA5 AmpR | This study |
| pJAF34 | SXI1α URA5 AmpR | This study |
| pJAF35 | SXI2aURA5 AmpR | This study |
a
Serotype A, congenic to H99 and KN99a.