FIG. 4.

PKC1 is important for protection against both oxidative and nitrosative stresses. The wild-type KN99a, the pkc1Δ, and the complemented pck1Δ::PKC1 strains were grown overnight in YPD containing 1 M sorbitol at 30°C and then diluted to 5 × 10⁷ cells/ml in PBS containing 1 M sorbitol. Five-microliter portions of 10-fold serial dilutions were plated onto YNB plates containing 1 M sorbitol plus the indicated oxidative or nitrosative stressor and grown for 3 days at 30°C. All plates were at pH 4.0.