FIG. 4.

Effect of Ca²⁺ and Ca²⁺ chelators on the M-Y transition of P. brasiliensis. Morphology of fungus during the M-Y transition at 37°C after 24 h (left panels) and 72 h (right panels) in the absence (A and B) or presence of 1 mM EGTA (C and D), 5 μM BAPTA (E and F), or 5 mM CaCl₂ (G and H). Arrows indicate no hyphal tip enlargement (E), hyphal tip enlargement (G), or hyphal tip enlargement plus buds or yeasts (H). Scale bar = 10 μm. (I) Graphical summary of the rate of morphological changes during hyphal tip progression through the M-Y transition stages at 24, 48, 72, and 120 h in the absence (CTR) or presence of calcium chelators (1 mM EGTA or 5 μM BAPTA) or 5 mM CaCl₂.