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. 2008 Sep 5;7(10):1856–1864. doi: 10.1128/EC.00110-08

FIG. 5.

FIG. 5.

Effect of temperature and calcineurin inhibitors on intrahyphal calcium levels. Fluorescence of mycelia after incubation with 3 μM Fluo-3 for 15 min at 25°C (A), for 15 min after the temperature shift to 37°C (B), or for 15 min after the temperature shift to 37°C preceded by incubation with 5 μM BAPTA for 20 min (C). Fluorescence of actively growing yeasts cultured at 37°C for 48 h and incubated for 15 min with 3 μM Fluo-3 in the absence (D) or presence of 2 μg/ml CsA (E) or 2 μg/ml FK506 (F). Fluorescence of cells at the M-Y transition cultured at 37°C for 48 h and incubated for 15 min with 3 μM Fluo-3 in the absence (G) or presence of 2 μg/ml CsA (H) or 2 μg/ml FK506 (I). Exposure times were the same for all experimental groups (4 s). Scale bar = 25 μm.