FIG. 6.

Relative CCH1 gene expression of P. brasiliensis by real-time RT-PCR. Mycelia (M) at 25°C, yeast (Y) at 37°C, or fungus at the M-Y transition at 37°C was cultured for 48 h either in the absence (black bars) or presence (white bars) of 2 μg/ml CsA before mRNAs were extracted. Bars indicate the means ± standard errors of representative experiments made in triplicate. P was <0.01 for CsA versus the control untreated yeast.