FIG. 8.

Effect of calcineurin inhibitors on MDR pumps. Mycelium (A to D) or yeast (E to H) cells were incubated for 20 min at 25°C or 37°C, respectively, in the absence or in the presence of 2 μg/ml CsA or 2 μg/ml FK506, followed by a 20-min incubation with 15 μM rhodamine 123. (A and E) Unlabeled fungi (fluorescence control); (B and F) with rhodamine 123 only; (C and G) with both CsA and rhodamine 123; (D and H) with both FK506 and rhodamine 123. Exposure times were the same for all experimental groups (4 s). Scale bar = 15 μm.