FIG. 11.
TNF-α responses of murine bone marrow-derived macrophages to hyphae of the wild type (bar A), the Δafmnt1 mutant (bar B), and the complemented mutant (bar C) are shown in panel A. Results obtained after stimulation with 100 ng lipopolysaccharide/well and with an unstimulated control are shown in bars D and E, respectively. (B) Results obtained in a mouse model of infection. CD-1 mice were infected retroorbitally with 1 × 106 conidia of the Δafmnt1 mutant (n = 11), the parental strain Afs35 (n = 12), and the complemented mutant Δafmnt1 afmnt1 (n = 13). The survival of mice is depicted over time.