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. 2008 Aug 1;7(10):1674–1684. doi: 10.1128/EC.00151-08

FIG. 5.

FIG. 5.

The dileucine motif in the C terminus of Dtr1p plays a role in PSM localization. (A) Sequence of the C-terminal tail of Dtr1p. The dileucine motif (blue) and the threonine (red) predicted to be phosphorylated are indicated. (B) Localization of mutant Dtr1 proteins during sporulation. Dtr1LAp-GFP, Dtr1T568Ap-GFP, Dtr1LA-T568Ap-GFP, and Dtr1LA-C10Δ-GFP were expressed in wild-type (WT) cells harboring MPC54-RFP (Y7749, Y7735, Y7784, and Y7884, respectively). The threonine-to-alanine (T568A) mutation did not affect PSM localization, while the dileucine mutation to alanine (LA) showed partial defects in PSM localization. The combination of the T568A and LA mutations showed an identical localization pattern to that of the LA mutant, while the LA-C10Δ mutation showed defects similar to those with the C10Δ mutation (Fig. 3). (C) Vegetative localization of mutant Dtr1p-GFPs at PM. Strains: LA, Y7752; T568A, Y7612; LA-T568A, Y7803; LA-C10D, Y7889. Bars, 5 μm.