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. 2008 Aug 1;7(10):1674–1684. doi: 10.1128/EC.00151-08

FIG. 6.

FIG. 6.

The C-terminal region is partially sufficient for Dtr1p localization to the PSM. Strains harboring the indicated chimeras were incubated on YPAD solid medium (vegetative cells) (A) and SPO solid medium (sporulating cells) (B). During both vegetative growth and sporulation, Vps10p-GFP showed the typical pattern for endosome/Golgi proteins and Vps10CΔp-GFP was found at vacuoles. Vps10CΔDtr1C16p-GFP showed vacuolar signals with some cytoplasmic puncta and diffuse staining in the cytoplasm of vegetative cells, with a relatively lower intensity of vacuolar signals in sporulating cells. Vps10TMΔCΔ-Dtr1TM-C10p-GFP was observed in vacuoles in vegetative cells and at PSMs as well as in vacuoles in sporulating cells. Vps10TMΔCΔ-Dtr1TM-GFP was observed in vacuoles in vegetative and sporulating cells. Strains: Y7449, VPS10-GFP; Y7451, VPS10CΔ-GFP; Y7750, VPS10CΔ-DTR1C16-GFP; Y8007, VPS10TMΔCΔ-DTR1TM-C10-GFP; and Y8190, VPS10TMΔCΔ-DTR1TM-GFP. Bars, 5 μm.