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. 2008 Aug 8;7(10):1742–1749. doi: 10.1128/EC.00169-08

FIG. 5.

FIG. 5.

Disruption of the chromosomal copy of the YlFBP1 gene and analysis of the disruptants. Scheme of the chromosomal region around YlFBP1 in wild-type strain P01a (a) and in strains RJM001 Ylfbp1::YlURA3 (b) or RJM002 Ylfbp1::YlLEU2 (c). The bold lines show the regions of DNA used for chromosomal disruptions. The NcoI site marked with an asterisk was created by PCR. (d) Southern analysis of the disruptants. Genomic DNA was digested with DraI. The 1,108-bp DNA fragment indicated as a probe was obtained by PCR. The sizes of the DNA bands are indicated. (e) Western blot analysis of YlFbp protein in the Ylfbp1::LEU2 disruptant. Extracts were subjected to PAGE and probed with a specific antibody against YlFbp. A loading control probed with an antibody against Y. lipolytica GAPDH is shown. WT, wild type.