FIG. 3.

Cao1 but not Cao2 catalyzes the oxidative deamination of ethylamine. (A) Four isogenic fission yeast strains (cao1⁺ cao2⁺, cao1Δ cao2⁺, cao1⁺ cao2Δ, and cao1Δ cao2Δ strains) were analyzed for the presence of CAO activity by use of a chemiluminescence activity assay with ethylamine (10 mM) as a substrate (top). As an internal control, aliquots of total protein extracts were analyzed by immunoblotting using an anti-PCNA antibody (bottom). (B) Total RNA was prepared from aliquots of cultures used in the experiment described for panel A. Representative RNase protection assays of cao1⁺ (top) and cao2⁺ (bottom) are shown, indicating steady-state mRNA levels. Actin (act1⁺) mRNA levels were probed as an internal control. NS, nonspecific signal.