FIG. 9.

S. pombe Atx1 interacts with Cao1 by two-hybrid assay. (A) Schematic representation of the LexA DNA binding domain alone or fused upstream of and in frame to the full-length Cao1 coding region. The indicated bait molecule was coexpressed with the VP16 activation domain or the VP16-Atx1 fusion protein. (B) Interactions between the proteins were detected by liquid β-galactosidase assays. The values are the averages from triplicate determinations ± standard deviations. (C) Total-cell-extract preparations from aliquots of cultures used in the assays described for panel B were analyzed by immunoblotting with anti-LexA, anti-VP16, or anti-PGK (as an internal control) antibody.