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. 2008 Aug 29;7(10):1865–1873. doi: 10.1128/EC.00125-08

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FIG. 3. — SPC enrichment. (A) Transmission electron micrograph of chemically fixed SPC-enriched fraction with isolated SPCs encircled. Bar, 1 μm. (B) SDS-PAGE analysis of the subcellular fractions of R. solani obtained after isopycnic centrifugation. Lanes 1, fraction on top of 30% sucrose layer; 2, fraction on top of 50% sucrose layer; 3, fraction 50% sucrose layer; 4, fraction on top of 70% sucrose layer (SPC-containing fraction); 5, SPC-enriched fraction (SPC-containing fraction treated with 2% Triton X-100). The arrow indicates the position of SPC18 protein. Numbers at left are molecular masses.