Figure 4.

Confocal optical sections of double-labeled cells. (A) Cells synchronized in G1 mainly contained cleavage bodies (green) that overlapped with coiled bodies (red; also see enlarged area). (B) Cells synchronized in S phase contained many cleavage bodies (green) adjacent to coiled bodies (red; also see enlarged area). (C) Cells synchronized in late S phase and G2 phase contained irregularly shaped cleavage bodies (green) adjacent to coiled bodies (red; also see enlarged area). (D) In situ hybridization showed that histone gene loci (red) are preferentially found adjacent to coiled bodies (green) in human T24 cells. (E) Histone gene loci (red) were regularly found colocalizing with cleavage bodies (green), specifically in S phase. (F) This series of optical sections show that the histone gene loci (red) are inside the cleavage bodies (green). (G) The U2 snRNA gene loci (red) were also often found adjacent to coiled bodies, but they were only found adjacent to cleavage bodies (green) or at a small distance from a cleavage body (H). Bar: A–E and G, 2 μm; F, 1 μm.