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. 1999 Nov;10(11):3825–3834. doi: 10.1091/mbc.10.11.3825

Figure 1.

Figure 1

Mitotic clonal analysis of TER mutant allele. (A) Shown is an example of an ovariole dissected from a P[hsFLP]; P[FRT]2R-G13 l(2)k15502/P[FRT]2R-G13 [arm-LacZ]. Ovaries were reacted with anti-Spc antibodies (green) and LacZ antibodies (red) to detect recombination-induced clonal egg chambers that would appear LacZ negative. The inset shows the effects of mitotic recombination on somatic cells by showing the loss of scutellar bristles in a P[hsFLP]; P[FRT]2R-G13 l(2)k15502/P[FRT]2R-G13 [arm-LacZ]. (B) Shown is an ovariole from a P[hsFLP]; P[FRT]2R-G13 P[arm-lacZ]/P[FRT]2R-G13 P[ovoD1; w+]. Most egg chambers manifest the ovoD1 phenotype, but the stage 13 egg chamber shows that FRT-dependent recombination can produce wild-type follicles under the experimental conditions.