Figure 5.

TER94 does not accumulate in stem cell fusomes and fusomes in bam mutant germ cells. Tumorous ovaries isolated from bam females were incubated with rat TER94 antibodies and monoclonal anti-Hts antibodies. (A) TER94 is uniformly distributed in the cytoplasm of bam mutant germ cells. Note that TER94 is not significantly enriched in any subcellular region. (B) This confocal section shows the positions of stem cell fusomes and fusomes in the same bam germarium. (C and D) A high-magnification view of the distribution of TER94 and Hts, respectively, in the anterior end of the germarium in A and B. The arrows indicate stem cell fusome positions; note that TER94 is not enriched at these positions. (E) This panel presents the merged projection of TER94 (red) and Hts (green) in the germarium’s anterior end. Stem cell fusomes do not show immunofluorescent overlap, indicating that TER94 does not become enriched.