Figure 10.

Colocalization of aFGF with markers for different intracellular organelles. (A and B) FGFR4-transfected COS cells were either untreated (A) or pretreated for 30 min with brefeldin A (B), incubated for 2 h at 37°C with CY3-aFGF, and then fixed and stained for the Golgi marker β-cop. (C and D) COS cells were cotransfected with the human transferrin receptor and FGFR4. The cells were then either left untreated (C) or pretreated for 30 min with 2 μg/ml brefeldin A (D) and incubated with CY3-aFGF for 2 h at 37°C. Subsequently, alexa-transferrin was bound to the cells at 4°C, and then the cells were washed and incubated for 20 min at 37°C and fixed.