Figure 11.

Effect of nocodazole on the intracellular localization of aFGF and FGFR4. COS cells transfected with FGFR4 (A–C) or cotransfected with FGFR4 and human transferrin receptor (D) were either left untreated (A) or pretreated for 30 min with 33 μM nocodazole (B–D). In some cases (A and B), the cells were then incubated with CY3-aFGF for 2.5 h at 37°C, fixed, and stained for tubulin. In C, the cells were stained with anti-FGFR4 and anti-tubulin. In D, the cells were treated with CY3-aFGF at 37°C for 2 h, alexa-transferrin was bound at 4°C, and then the cells were incubated for 20 min at 37°C.