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Kinetics of aFGF-induced disappearance of FGFR4 from the caveolin-rich fraction. COS cells transfected with FGFR4 were grown for 12 h in the absence of serum and then incubated in the presence of 50 ng/ml aFGF for the indicated periods of time. Caveolin-rich fractions were prepared as described in Figure 2. Fractions 4 and 5 were pooled, dialyzed against distilled water, lyophilized, separated by SDS-PAGE, and visualized by immunoblotting with anti-FGFR4 antibodies.
