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Stimulation of tyrosine kinase activity in caveolin-rich fractions of FGFR4-transfected COS cells. The cells were grown in the absence of serum for 12 h and then incubated in the presence of 50 ng/ml aFGF for 10 min. The cells were fractionated as in Figure 2B, and the fractions were analyzed by SDS-PAGE and immunoblotted with anti-phosphotyrosine antibodies.
