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Colocalization of aFGF with EEA1, a marker for early endosomes. FGFR4-transfected COS cells were incubated with CY3-aFGF for 8 min (A) or 2 h (B and C) at 37°C (A and B) or 16°C (C) and then fixed and stained with anti-EEA1 antibodies followed by FITC-conjugated secondary antibody.
