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. 1999 Nov;10(11):3849–3862. doi: 10.1091/mbc.10.11.3849

Figure 2.

Figure 2

Deletion of SRO9, SLF1, and LHP1 does not result in synthetic lethality. (A) An sro9::URA3/SRO9 diploid (YSS203) was sporulated, and tetrads were dissected. The sro9::URA3 allele confers a slight slow-growth phenotype, which segregates with the uracil auxotrophy. Two of the smaller URA3 segregants are indicated with small arrowheads, and two of the larger ura3 segregants are indicated with large arrowheads. (B) Segregants of the tetrad dissection of an slf1::HIS3/SLF1, lhp1::LEU2/LHP1 diploid (YSS222). The deletion of SLF1 or LHP1 or both did not confer a growth defect. (C) Segregants of the tetrad dissection of an sro9::URA3/SRO9, slf1::HIS3/slf1::HIS3, lhp1::LEU2/LHP1 diploid (YSS227/YSS228). sro9::URA3, slf1::HIS3, lhp1::LEU2 triple deletion segregants grew similarly to sro9::URA3 haploids, indicating that the additional deletions did not confer synthetic lethality or slow growth. Two large and two small segregants are indicated as in A.