Table 2.
Specificity and efficiency of PLD antibodies
| PLD activity (pmol/30 min)a
|
||
|---|---|---|
| PLD1-expressing Sf9 cellsb | PLD2-expressing Sf9 cells | |
| Total in lysate | 17.8 ± 0.1 | 195 ± 7 |
| IP/PLD1 | 16.9 ± 0.2 (95%) | 0.9 ± 0.1 |
| IP/PLD2 | 0.09 ± 0.003 | 172 ± 5 (88%) |
Parentheses indicate the percentage activity immunoprecipitated of the total activity.
a
PLD activity was measured using l-dipalmitoyl phosphatidyl [methyl-3H] choline following the method of Brown et al. (1993). Activity is expressed as the means ± SD of triplicate determinations.
b
PLD1 assayed in the presence of 6 μM Arf preactivated with GTPγS.