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. 1999 Nov;10(11):3891–3908. doi: 10.1091/mbc.10.11.3891

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Copyright © 1999, The American Society for Cell Biology

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Syntaxin 7 is a broadly expressed SNARE. (A) A rabbit polyclonal anti-syntaxin 7 antibody recognizes a major 39-kDa band in HeLa cell PNS (control). This band represents endogenous syntaxin 7, because it can be blocked with 1 μg/ml recombinant syntaxin 7 (syntaxin 7) but not 1 μg/ml recombinant syntaxin 13 (syntaxin 13). (B) PNSs from Jurkat, CHO, HepG2, Madin–Darby canine kidney, Cos7, and 293 cell lines were analyzed using the anti-syntaxin 7 antibody. A single major 39-kDa band is detected in every cell line investigated.