Figure 7.

Immunoprecipitation of YT521-B protein complexes. YT521-B or its deletion variants were expressed as EGFP fusion proteins and precipitated with anti-EGFP antibodies. Immunocomplexes were analyzed by Western blot after SDS-PAGE using the antibodies indicated. The endogenous coprecipitating proteins were detected in all experiments. CL, crude lysates; IP, immunoprecipitation. The analysis of the immunoprecipitates is shown on the left. The reblot using anti-GFP antibody to demonstrate proper protein expression is shown on the right. (A) Blot with anti-Sam68. (B) Blot with anti-SAF-B. (C) Blot with mAb104 that recognizes SR proteins. (D) Blot with anti-htra2-beta1. The closed arrow indicates the location of the dephosphorylated protein, whereas the open and striped arrows show the phosphorylated and hyperphosphorylated forms (Daoud et al., 1999).