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. 1999 Nov;10(11):3909–3926. doi: 10.1091/mbc.10.11.3909

Figure 9.

Figure 9

Localization of YT521-B, Sam68, and SAF-B. BHK cells were transfected, fixed, and incubated with the antibodies indicated. The staining in green (left column) is always EGFP-YT521-B. The staining in red (middle column) is either Sam68 or SAF-B, as indicated. The pictures in the right column show the overlay of the red and green signals. Bar, 5 μm. (A) Transfection of EGFP-YT521-B; endogenous Sam68 (red) was detected. (B) Transfection of EGFP-YT521-B and p59fyn; endogenous Sam68 (red) was detected. (C) Transfection of EGFP-YT521-B and p59fynKA; endogenous Sam68 (red) was detected. (D) Transfection of EGFP-YT521-B and FLAG-SAF-B; FLAG-SAF-B was detected with anti-FLAG (red). Arrows indicate colocalization of SAF-B and YT521-B at the periphery of YT521-B nuclear dots.