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. 2008 Oct 27;3(10):e3530. doi: 10.1371/journal.pone.0003530

Figure 4. Functional transcriptional effects of Fbxl3 and Fbxl21.

Figure 4

A. oFbxl3 and oFbxl21 dose-dependently reverse oCRY1 inhibition of oCLK/oBM1 induced transactivation of an oRev-erb α reporter construct. Transfection conditions per well: 100 ng of oClock, 100 ng of oBmal1, 100 ng of oCry1, 100 or 200 ng of each Fbxl or ev, 50 ng of oRev-erb alpha promoter reporter, 50 ng of b-Gal reporter and/or necessary amount of empty vector to make a final amount of 600 ng. B. mFbxl21 dose-dependently reverses mCRY1 inhibition of mCLK/mBM1 induced transactivation of an mPer1 reporter construct. Transfection conditions per well: 100 ng of mClock, 100 ng of mBmal1, 20 ng of oCry1, 100 or 200 ng of mFbxl21 or ev, 50 ng of Per1 promoter reporter, 50 ng of b-Gal reporter and/or necessary amount of empty vector to make a final amount of 600 ng. Data in A/B are mean±SEM of one triplicate representative from 3 replicate experiments. Different letters above bars indicate significantly different groups (p<0.05).