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. 2008 Oct 27;3(10):e3517. doi: 10.1371/journal.pone.0003517

Figure 6. Lateral expansion of Sonic hedgehog expression and flattening of midbrain ventricle, associated with loss of N-cadherin in Wnt5a−/− embryos.

Figure 6

(A) Shh, Foxa2 and Lmx1a are laterally expanded by E11.5 in Wnt5a−/− mice. The invagination at the medial hinge-point is markedly reduced/flattened in the mutants. Red bars delimit the alar plate (AP), basal plate (BP), floor plate (FP) and roof plate (RP). Scale bar 250 µm. (B) The flattened VM invagination was quantified by measuring the angle formed between the midline and the ventricular wall at several levels throughout the VM of E10.5 mice, when this morphological change first became obvious. A significant difference was found in the Wnt5a−/− mice forming a U-shaped ventricle, with an angle of 59.4°±2.8° from the midline as compared to 49.1°±4.3° in WT mice. (C) Western blot of E9.5 whole brain of WT and Wnt5a−/− mice revealed a marked reduction of N-cadherin in Wnt5a−/− mice.