Expanded m/z region showing the 18+ charge state in the ESI FT-ICR mass spectra of horse heart myoglobin. (A) Mb (1 μM) in water/methanol (1/1, v/v, 1% acetic acid). (B) Iodinated Mb (1 μM) in water/methanol (1/1, v/v, 1% acetic acid), electrochemical iodination at 0.4 V vs SCE for 1 min, 20 mM KI, (C) Iodinated Mb (1 μM) in water/methanol (1/1, v/v, 1% acetic acid), electrochemical iodination at 0.4 V vs SCE for 5 min, 20 mM KI, (C) Iodinated holoMb (20 μM) in water/methanol (1/1, v/v, 1% acetic acid), electrochemical iodination at 0.4 V vs SCE for 10 min, 20 mM KI.