Figure 3.

Molecular mass distribution and elution profile of oxidized and reduced αBT162C protein. Protein (0.1 mg each) in buffer was injected into a TSK5000 gel filtration column connected to a multi-angle light scattering instrument, and the data was analyzed as described under Methods. Solid line and filled circle, oxidized αBT162C; broken line and unfilled circle, reduced αBT162C. A broad elution peak for the oxidized αBT162C protein suggests that the cysteine mutant forms heterogeneous aggregates with wide-range of molecular species upon oxidation.