Table 1.
Effects of different Ubcs on progression through mitosis
| Injection | Average (SD) | Range | Number of cells |
|---|---|---|---|
| Buffer | 8.7 (4.2) | 6–11.5 | 8 |
| Wild-type UbcH10 | 9.9 (4.1)a | 5–18.5 | 19 |
| Mutant UbcH10 | 53.8 (17.2)b | 25–104 | 37 |
| Mutant UbcH5b | 17.7 (5.13)a | 11–28 | 14 |
| Mutant Ubc3/CDC34 | >100c | >100c | 17 |
a Not significantly different from cells injected with buffer.
b Significantly different from other values; P < 0.001.
c
Eleven of 17 cells did not established a metaphase plate during observation period (120 min); six cells arrested at metaphase.