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Substrate inhibition is observed when the gp44/62 steady-state ATPase rates are measured in the presence of the gp45 clamp. The steady-state ATPase rates of gp44/62 in the presence (A) and in the absence (B) of gp45 were measured using an ATP regeneration system assay method as described under “Experimental Procedures.” Component concentrations were 1 μm gp44/62 and 1.5 μm gp45 (A) and 15 μm gp44/62 and no gp45 (B). Total input ATP concentrations are plotted on the x axis.
