TABLE 2.

Enzyme activities of control enzymes in some of the pyruvate carboxylase-deficient cell lines with the lowest glucose-stimulated insulin release

Enzyme rates are expressed as a percentage of the control parent INS-1 832/13 cell line equal to 100%. Average specific enzyme activities defined as 100% in nmol of product formed/min/mg of protein were 4 for propionyl-CoA carboxylase, 56 for malic enzyme, 89 for NADP isocitrate dehydrogenase, 167 for ATP citrate lyase, and 953 for aspartate amino transferase.

Enzyme	Relative enzyme activity
	PC118	PC1971	PC1973	PC118/1971
	% of INS-1 832/13
Propionyl-CoA carboxylase	88	89	86	106
Malic enzyme	75	99	135	103
NADP isocitrate dehydrogenase	117	94	104	105
ATP citrate lyase	102	94	81	112
Aspartate aminotransferase	126	120	99	109