TABLE 3.

Decreased carbon incorporation from glucose into lipid and no decrease in glucose oxidation to CO₂ in pyruvate carboxylase-deficient cells

Cell lines stably transfected with shRNA targeting the pyruvate carboxylase mRNA or a control vector (U6) were incubated for 45 min with 16.7 mm [U-¹⁴C]glucose (specific radioactivity 0.75 mCi/mmol glucose). Radioactivity incorporated into a lipid extract and CO₂ was measured. Results are the mean ± S.E. of four replicate incubations for each condition.

Cell line	Glucose incorporation
	Into lipid fraction	Into CO2
	nmol/mg cell protein
U6 (control)	0.92 ± 0.06	2.2 ± 0.1
PC118/1971	0.73 ± 0.04a	2.0 ± 0.1
PC118/3064	0.64 ± 0.01b	2.2 ± 0.1
PC118/1973	0.65 ± 0.01b	2.4 ± 0.1

^ap < 0.05 versus control.

^bp < 0.005 versus control.