FIGURE 7.
miRNA qPCRs. A and B show the dissociation temperature curves for miR-186 (A), miR-150 (B), and the positive control U6 snRNA (lysates from hEVEC cells). C, levels of miR-186 and miR-150 in hEVEC and HeLa cells (qPCR, means ± S.D., two experiments in triplicate, normalized to U6 RNA). *, p < 0.001 compared with hEVEC. D, levels of miR-186 and miR-150 in normal and cancerous human endometrial tissues. Discarded surgical uterine tissues were obtained from two patients with endometrial endometrioid (type I) adenocarcinomas. In each case fresh samples were collected from a site that included the cancerous tissue and from an adjacent macroscopic (and histologically confirmed) normal endometrial site. For each case, qPCR Ct data of miR-186 and miR-150 levels in cancer tissues were normalized to those in the normal tissue of the same case.