Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Oct 17;283(42):28729–28740. doi: 10.1074/jbc.M802751200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Shown are 4pX-1 (A) and 4pX-1-geminin^kd (D) cells synchronized in G₁/S by the double thymidine block, immunostained for BrdUrd (BrdU) incorporation (green) and phospho-H3 (red) in the indicated time course after release from the block with (+) or without (-) pX expression. pX expression was initiated by tetracycline removal 10 h before release from the second block and continued for the indicated time course. BrdUrd was added 30 min before fixation. B, confocal microscopy of BrdUrd-positive and phospho-H3-positive cells from the 10.5-h time point with pX expression. C and E, 4pX-1 (C) and 4pX-1-geminin^kd (E) cells, quantification of three independent experiments of BrdUrd-positive (green) and phospho-H3-positive (red) cells by ImageJ software, counting at least 1000 cells for each histogram.