TABLE 2.
Phosphorylation of Tyr47 and Tyr58 in activated FcRγ gel bands
|
FcRγ gel band
|
Ion peak area (×10−8)
|
Calculated molar ratios (Tyr(P)/Tyr peptide)
|
||||
|---|---|---|---|---|---|---|
| FcR 43-53 (Tyr(P)47) | FcR 43-53 | FcR 54-62 (Tyr(P)58) | FcR 54-62 | FcR 43-53 (Tyr(P)47/Tyr47) | FcR 54-62 (Tyr(P)58/Tyr58) | |
| 1 | 5.7 | 3.4 | 1.4 | 6.7 | 1.9 | 0.4 |
| 7.3 | 2.7 | 1.8 | 4.5 | 2.9 | 0.7 | |
| 2 | 5.4 | 8.2 | 1.5 | 6.7 | 0.8 | 0.5 |
| 5.5 | 6.4 | 0.8 | 10.8 | 1.0 | 0.2 | |
| 3 | 0.2 | 39.8 | 0.02 | 8.8 | <0.01 | <0.01 |
| 1.1 | 8.1 | 0.3 | 29.2 | 0.14 | 0.02 | |
| 1.2 | 25.4 | 0.1 | 43.7 | 0.05 | <0.01 | |
| 4 | 0.2 | 41.0 | 0.1 | 67.2 | <0.01 | <0.01 |
| 0.7 | 25.0 | 0.7 | 38.3 | 0.03 | 0.03 | |
| 0.8 | 28.9 | 0.3 | 46.7 | 0.03 | <0.01 | |