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. 2008 Oct 17;283(42):28361–28369. doi: 10.1074/jbc.M805059200

FIGURE 2.

FIGURE 2.

Growth and consumption of free Met sulfoxides by SK-Hep1 cells. A, growth of SK-Hep1 hepatocytes on Met and Met sulfoxide media. The cells were grown in Met-free DMEM supplemented with 0.1 mm Met (diamonds), 0.1 mm Met-RO (triangles), 0.1 mm Met-SO (circles), or 0.1 mm Met-RSO (squares). Cell growth was quantified in a cell proliferation assay at 0, 24, 48, 72, and 96 h. The error bars represent the standard deviations from five independent experiments. B, consumption of Met in Met medium (diamonds), Met-SO in Met-RSO medium (squares), Met-RO in Met-RSO medium (triangles), Met-SO in Met-SO medium (circles), and Met-RO in Met-RO medium (stars) at 0, 24, 48, 72, and 96 h. The initial concentration of each amino acid was 0.1 mm. The error bars represent the standard deviations from three independent experiments.