TABLE 1.
Deduced functions of open reading frames in the tautomycin biosynthetic gene cluster
| Gene | Sizea | Proposed function | Homologb | Identity/similarity |
|---|---|---|---|---|
| %/% | ||||
| orf(−3) | 237 | Dehydrogenase | FabG (1Q7C_A) | 47/64 |
| orf(−2) | 589 | Flavohemoprotein | Hmp (CAM78118) | 25/35 |
| orf(−1) | 231 | Unknown | YedU (1ONS_A) | 22/31 |
| Predicted upstream boundary of the ttm cluster | ||||
| ttmF | 429 | Transcriptional activator | StaR (BAC55205) | 12/18 |
| ttmG | 176 | Decarboxylase | Plav_1659 (YP_001412935) | 30/50 |
| ttmH | 5770 | Polyketide synthase (modules 10-12) | ||
| ttmI | 5710 | Polyketide synthase (modules 7-9) | ||
| ttmJ | 10827 | Polyketide synthase (loading module and modules 1-6) | ||
| ttmK | 456 | Esterase | PnbA (1QE3_A) | 29/44 |
| ttmL | 190 | Unknown | Ybhb (1FUX_A) | 23/30 |
| ttmE | 361 | Glyceryl transferase/phosphatase | FkbH (AAF86387) | 67/77 |
| ttmA | 373 | Acyl CoA dehydrogenase | FkbI (1R2J_A) | 62/73 |
| ttmD | 84 | Acyl carrier protein | FkbG (AAF86389) | 57/69 |
| ttmB | 289 | β-Hydroxyacyl CoA dehydrogenase | FkbK (AAF86390) | 56/68 |
| ttmC | 218 | O-Methyl transferase | FkbG (AAF86386) | 61/75 |
| ttmM | 353 | α-Ketoglutarate-dependent hydroxylase | XanA (XP_659714) | 23/34 |
| ttmN | 368 | Unknown | EhpF (AAN40895) | 35/51 |
| ttmO | 314 | Citryl CoA lyase | CitE (1Z6K_A) | 25/36 |
| ttmP | 407 | CoA transferase | CaiB (1XVV_A) | 24/41 |
| ttmQ | 351 | Transcriptional activator | StrR (CAH94333) | 36/48 |
| ttmR | 467 | Dehydratase | PrpD (2HP3_A) | 19/30 |
| ttmS | 274 | Cyclase | PFL_4035 (YP_261132) | 26/40 |
| ttmT | 257 | Thioesterase | PikAV (AAC69333) | 41/52 |
| Predicted downstream boundary of the ttm cluster | ||||
| orf1 | 90 | Unknown | ||
| orf2 | 195 | Unknown | ||
| orf3 | 129 | Unknown |
a
The numbers are in amino acids.
b
Given in parentheses are NCBI accession numbers.