Interaction between MyoGEF and Plk1. A, HeLa cells were
transfected with plasmids encoding Myc-Plk1 and GFP-MyoGEF.
Anti-Myc-conjugated agarose was used to precipitate Myc-Plk1 from the
transfected cell lysate, followed by immunoblotting with an antibody specific
for GFP. B, schematic diagram of MyoGEF fragments. The numbers
indicate the amino acids. C, a plasmid encoding GFP-Plk1 was
cotransfected into HeLa cells with empty vector or plasmids encoding
Myc-tagged MyoGEF fragments (71–388, 71–565, 392–565, or
392–780). Anti-Myc-conjugated agarose was used to precipitate Myc-tagged
MyoGEF fragments from the transfected cell lysate, followed by immunoblotting
with an antibody specific for GFP. D, a plasmid encoding GFP-MyoGEF
was cotransfected into HeLa cells with empty vector or a plasmid encoding
Myc-PBD. Anti-Myc-conjugated agarose was used to precipitate Myc-PBD from the
transfected cell lysate, followed by immunoblotting with an antibody specific
for GFP. wb, Western blot; IP, immunoprecipitation.