FIGURE 5.
Mutations of ASF affect its subcellular location and role in tau exon 10 inclusion. a, subcellular location of ASF mutants. HA-tagged ASFS3A or ASFS3D was overexpressed in HeLa cells, and then the cells were fixed and immunostained with anti-HA and FITC-labeled secondary antibody. Hoechst was used for nucleus staining. b, Dyrk1A could not drive ASFS3A into speckles. HA-tagged ASFS3A (red) was co-expressed with Dyrk1A (green) in HeLa cells and immunostained as described above. c, effects of ASF mutations on tau exon 10 splicing. The mini-tau gene was co-transfected with ASFS3A or ASFS3D into COS7 cells, and tau exon 10 splicing was analyzed by RT-PCR after a 48-h transfection. *, p < 0.05 versus control (Con) group.